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Polymer gel cartridge column Purif™-StarGel series

Summary

Polymer gel is well-known as ion exchange resin in the past and as stationary phase for biochemical field in the present. Polymer gel has the wide range of usable pH and high chemical stability, so it can be used in combination with conventional packed columns for middle pressure.

Features

  • There are 6 kinds of gel in total lineup.
  • 2 kinds of particle size and 3 kinds of polarity (high, middle, low)
  • Wide range of usable pH:
    • low and middle polarity gel：pH1-14
    • high polarity gel：pH2-12
  • Separation conditions can be examined with a small amount of gel.
  • Test kit is available to select a suitable gel.
  • These columns can be handled as same as conventional Purif™-Pack series.
  • These columns must be an option if you have problems with conventional columns from adsorption/desorption of sample to chromatography.
  • These columns show high performance as a preliminary column for the separation of natural products extract when combining with our Flash mass instrument "Purif™-Rp2".
  • In the separation by reverse phase mode, samples are eluted with low polar solvent and it is easy to concentrate sample compared with the separation by ODS column.
  • Trusted polymers made by Mitsubishi Chemical Co., Ltd. are packed.

Application

The separation with Sirius-S (for column chromatography)

  • It can be used for conventional ODS column.
    ※Samples are eluted with low polar solvent and it is easy to concentrate samples compared with the separation by conventional ODS column.
  • Reduction of crystallization in the system

Separation conditions

Column
Sirius-S size20
Mobile phase
Water / Methanol (30/70)
Isocratic 70%
Gel weight
12g
Flow rate
10ml / min
Sample
1. Acetaminophen
2. Caffeine
Detection
UV254nm

image Comparison data(Purif™-Pack)-1

image Comparison data(Purif™-Pack)-2
※Comparison data(Purif™-Pack)

Other examples

Other examples
Column Subject compounds
Vega Water-soluble vitamins, Flavorings, Chinese medicines, Peptides, Proteins
Sirius Alkaloids, Phthalic acid esters, Polyene antibiotics, Peppers, Green tea Penicillin antibiotics
Antares Halogen compounds, Removal of low polar substances

How to use

How to use
Loading amount Indication amount: (5-10% of the volume of column)mg/ml
Loading method
  • Column packed small particle size gel: Injection with syringe (same as the method for Purif™-Pack)
  • Column packed large particle size gel: Injection with syringe, Dilute solution sample(sending by pump or connecting empty column)
  1. Wash with organic solvent (if necessary)
  2. Replace with water in the case of reverse phase mode (if necessary)(Flow at 40ml/min to remove air)
  3. Wash with solvent to be used

Photo 2ch Parallel Flash and high pressure liquid Chramatography Purif™-Rp2
Photo 2ch Parallel Flash and high pressure liquid Chramatography Purif™-Rp2

Points of column selection

  • Select to absorb the target substance or impurities in a sample
  • Select small particle size gel for chromatography
  • To purify samples which are eluted with high polar solvent in reverse phase mode
  • As a preliminary column to purify samples which have lots of contaminants
  • Antares provides specific separation against samples having plural halogen atoms
How to use
Product name Chemical structure Features Adsorption amount Polarity
Methacrylic synthetic adsorbent
Vega 		image Chemical structure
  • Effective to absorb high polar organic substances
  • Effective to absorb substances having Hydrogen bonding functional group(Carboxyl group, Ester group, Amino group, Amide group)
  • Possible to separate by normal phase chromatography
  • Effective to purify proteins
 Small High
Polystyrene synthetic adsorbent
Sirius 		image Chemical structure
  • The Standard of polymer synthetic adsorbents
  • Effective to absorb compounds having Benzene ring or conjugated double bond
  • Possible to separate by normal phase chromatography
  • Effective to purify proteins
 Medium Medium
Modified polystyrene synthetic adsorbent
Antares 		image Chemical structure
  • High absorption amount(possible to absorb much amount of high polar substances than Sirius)
  • Effective to purify hydrophilic compounds
  • Effective to purify halogen compounds
  • Possible to separate by normal phase chromatography
  • Effective to purify proteins
 Large Low

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